Phospho- stat3

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August undefined, 2024

WebUse Anti-phospho-STAT3 (Tyr705) Antibody, clone 9E12 (Mouse Monoclonal Antibody) validated in ELISA, IP, WB to detect phospho-STAT3 (Tyr705) also known as Acute-phase response factor or DNA-binding protein APRF. This Stat3 Antibody has been validated in Human, Mouse and Canine samples. Quality WebImmunogen: Phosphorylated Human Stat3 Peptide Application: Intracellular staining (flow cytometry) (Routinely Tested) Vol. Per Test: 20 µl RRID: AB_1645327 Storage Buffer: Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide. Regulatory Status: RUO View All Formats Preparation And Storage

Purified anti-STAT3 Phospho (Tyr705) Antibody - BioLegend

WebNational Center for Biotechnology Information WebMar 14, 2013 · Total and phospho–STAT3 was detected by Western blot. B: Healthy donor whole blood was pretreated with ATR-107 or control Ab for 30 minutes prior to stimulation with 10 ng/ml IL-21 for 15 minutes. Cells were fixed, permeabilized, stained with A647-labeled anti–phospho–STAT-3 antibody, and analyzed using flow cytometry gated on CD4 … grass valley storage unit

Phospho-STAT3 (Tyr705) Polyclonal Antibody - Thermo …

WebGet better batch-to-batch reproducibility with a recombinant antibody. Anti-STAT3 (phospho S727) antibody [E121-31] (ab32143) Research with … WebNov 15, 2002 · As shown in Fig. 3,A, phospho-Stat3 is detected in the nucleus with different intensities in all three of the human prostate cancer cell lines examined. The intensity of red stain (Nova-Red) corresponds to the level of active phospho-Stat3, whereas the blue stain (hematoxylin) is the counterstain. WebThe STAT3 (Tyr705)Phospho- Assay is a sandwich immunoassay (Figure 1). MSD provides a plate that has been pre-coated with capture antibody for STAT3total . The user adds the sample and a solution containing the detection antibody—anti-phospho-STAT3 (Tyr705) conjugated with an electrochemiluminescent grass valley support portal

On-target JAK2/STAT3 inhibition slows disease progression in …

Phospho-STAT3 (Tyr705) Recombinant Polyclonal Antibody …

WebApr 14, 2024 · Mass spectrometry–based analyses demonstrated that salt-inducible kinase 1 (SIK1) binds AKT and undergoes AKT-mediated phosphorylation, which compromises SIK1 tumor-suppressive functions. As a result, AKT relieved the binding and repression of STAT3 by SIK1 in a phosphorylation-dependent manner, resulting in breast cell … WebSTAT3 is tyrosine phosphorylated by receptor kinases in response to a variety of cytokines and growth factors. It forms homo- or heterodimer with STAT1 when tyrosine is phosphorylated, and then translocates to nucleus, acting as a transcription regulator. chloe sevigny and sinisa mackovicWebDec 21, 2012 · Western blotting for STAT3 revealed detectable phosphorylation of Y705 and S727 of STAT3 (Fig. 1 B and C) in a large panel of BTSC cultures with diverse mutational profiles (Table 1). Moreover, JAK2 and STAT3 phosphorylations were retained in orthotopic xenograft tumors initiated from BTSC lines (Fig. 1 A and B). chloe sevigny birth chart

"WebNitro-oleic acid (NO2-OA), pluripotent cell-signaling mediator, was recently described as a modulator of the signal transducer and activator of transcription 3 (STAT3) activity. In our … " - Phospho- stat3

Phospho- stat3

The role of STAT3 in autophagy - PMC - National Center for ...

WebThe BD™ CBA Phospho Stat3 (Y705) Flex Set is a bead-based immunoassay capable of measuring human signal transducer and activator of transcription 3 (Stat3) that has been tyrosne-phosphorylated at Tyr-705 in denatured cell lysate samples. Human reactivity was determined by testing cell lysates with the BD CBA Phospho Stat3 (Y705) Flex Set. WebPVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT3 (Y705) Monoclonal Antibody (Catalog # MAB4607) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005 ). A specific band was detected for Phospho-STAT3 (Y705) at approximately 95 kDa (as indicated). This experiment was conducted under …

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WebApr 11, 2024 · STAT3 is widely expressed in organs and tissues, and the phosphorylation of STAT3 motivates downstream genes related to proliferation and apoptosis, thus performing proliferation effects. A previous study has revealed that the motivation of STAT3 signaling promotes colonic mucus secretion in DSS-induced colitis in mice ( 28 ). WebA Life Sciences and Diagnostics business focused on enabling scientists and healthcare professionals to advance research, discovery and detection to improve global health Analytical & Enterprise Solutions An Analytical and Enterprise solutions business, including leading OneSource laboratory services, focused on accelerating scientific outcomes

WebPhospho-STAT3 was detected in immersion fixed IFN-alpha treated Daudi human Burkitt's lymphoma cell line using 10 µg/mL Human Phospho-STAT3 (Y705) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4607) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red ...

WebMay 7, 2015 · STAT3 (signal transducer and activator of transcription 3 [acute-phase response factor]) is a latent transcription factor that mediates extracellular signals such as cytokines and growth factors through interaction with polypeptide receptors at the cell surface.6STAT3 protein becomes transcriptionally activated primarily by tyrosine … WebMar 15, 2024 · The phosphorylation levels of JAK2 and STAT3 were significantly increased by shRNA-circ8199 compared with the control counterparts, which was reversed by the OGT inhibitor OSMI-1 (Figure 5B-D). Accordingly, the binding of OGT and JAK2 was enhanced when circ8199 was knocked down ( Figure 5E ).

WebMar 21, 2024 · STAT3 (Signal Transducer And Activator Of Transcription 3) is a Protein Coding gene. Diseases associated with STAT3 include Autoimmune Disease, Multisystem, Infantile-Onset, 1 and Hyper-Ige Recurrent Infection Syndrome 1, Autosomal Dominant . Among its related pathways are IL-9 Signaling Pathways and Prolactin Signaling .

WebApr 12, 2024 · This was evidenced by the fact that the reduced phosphorylation of STAT3 at the Y705 position by stattic has unleashed these pro-metastatic functions of the cancer cells, leading to the increased presence of CSCs and to an EMT-like phenotype in the cells. The negative regulatory roles of Y705-STAT3 on CSC generation was also evidenced by … grass valley surgery center caWebDec 1, 2024 · We further identified STAT3 in our extracted subnetwork and crucial proteins, indicating that several CSC-related proteins, including drug resistance markers (ABCG1, … grass valley surgery centerWebNitro-oleic acid (NO2-OA), pluripotent cell-signaling mediator, was recently described as a modulator of the signal transducer and activator of transcription 3 (STAT3) activity. In our study, we discovered new aspects of NO2-OA involvement in the regulation of stem cell pluripotency and differentiation. Murine embryonic stem cells (mESC) or mESC-derived … grass valley sugar hair removalWebSTAT3 belongs to the family of STAT (signal transducers and activators of transcription) proteins which are phosphorylated by receptor associated kinases, translocate to the … grass valley storage facilitiesWebSTAT3 Antibodies Antibodies that detect STAT3 can be used in several scientific applications, including Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and Flow Cytometry. These antibodies target STAT3 in Human, Mouse, Rat, Non-human primate and Porcine samples. grass valley superior courtWebPhospho-Stat3 (Tyr705) Antibody detects endogenous levels of Stat3 only when phosphorylated at Tyr705. The antibody does not cross-react with other Stat proteins … chloe sevigny exerciseWebJan 8, 2024 · N4 blocked STAT3 and phospho-tyrosine (pTyr) peptide interactions in fluorescence polarization (FP) assay, specifically abolished phosphor-STAT3 (Tyr705), and suppressed expression of STAT3 ... chloe sevigny age in kids