C11-bodipy and ferroorange staining

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August undefined, 2024

WebJul 27, 2024 · Ischemia-reperfusion (I/R) injury is a promising therapeutic target to improve clinical outcomes after acute myocardial infarction. Ferroptosis, triggered by iron overload and excessive lipid peroxides, is reportedly involved in I/R injury. However, its significance and mechanistic basis remain unclear. WebMay 13, 2024 · Tumor cells were seeded in six-well plates with glass bottoms before staining. For C11-BODIPY staining, 1 ml PBS containing 10 μM BODIPY 581/591 C11 …

FTH promotes the proliferation and renders the HCC cells …

WebAug 28, 2024 · Thereafter, lipid peroxidation was assessed by C11-BODIPY 581/591 probes through flow cytometry. b) Representative images of 4-HNE immunofluorescent staining (in red color) through fluorescent microscopy. DAPI was used to stain nuclei (in blue). Quantification of cellular 4-HNE fluorescence was shown in (c) (n = 3). WebC11-BODIPY probe and real time PCR was used to detect the ferroptosis level in breast cancer cells. FerroOrange probe and western blot was employed to measure ferritinophagy. The cell viability was examined by CCK-8, EdU and AM/PI double staining. Results dangerous country song

Immunohistochemistry of M1 and M2 alveolar macrophages

WebFeb 20, 2024 · FerroOrange staining and quantitative analysis by flow cytometry indicated a dose-dependent increase in the fluorescence intensities with increasing Fe … WebDec 21, 2024 · C11 Staining For the evaluation of lipid ROS generation in vitro, C11 staining by C11 BODIPY 581/591 kit (ThermoFisher, catalog # C10445) was used in our study. After the treatment, cell culture media was removed, and 10 μM Image-iT ® Lipid Peroxidation Sensor (Component A) was applied to the cells for 30 min at 37 °C. WebMar 24, 2024 · This protocol uses BODIPY™ 581/591 C11 (Life Technologies, D3861) as the reporter for lipid peroxidation. BODIPY-C11 can be used to detect reactive oxygen … dangerous countries to visit 2022

NRF2 controls iron homeostasis and ferroptosis through HERC2 …

Optimization of the C11‐BODIPY581/591 dye for the

WebOct 27, 2024 · Note also that live cell imaging showed a granular intracellular C11-BODIPY staining during non-canonical pyroptosis, in contrast to a homogenous staining pattern during ferroptosis. WebHere we describe a method to detect ferroptosis by determining the amount of lipid peroxides in cellular membranes using BODIPY-C11 probe and flow cytometry. … birmingham performers school birmingham perinatal mental health team

"WebFeb 1, 2024 · Collectively, this study not only offers basic knowledge regarding how NRF2 controls iron homeostasis but also indicates the translational value of inhibiting NRF2 to enhance the LIP and drive cancer cell death through ferroptosis. RESULTS NFE2L2/NRF2 deletion sensitizes ovarian cancer cells to ferroptosis " - C11-bodipy and ferroorange staining

C11-bodipy and ferroorange staining

Induction of ferroptosis in response to graphene quantum dots …

WebJul 30, 2024 · In this study, we found that IR induced ferroptosis in intestinal epithelial cells, as indicated by the increase in intracellular iron levels and lipid peroxidation, upregulation of prostaglandin-endoperoxide synthase 2 (PTGS2) mRNA, reduced glutathione peroxidase 4 (GPX4) mRNA and glutathione (GSH) levels, and significant mitochondrial damage. WebC11-BODIPY(591/581) staining was combined with flow cytometry measurements to obtain multiparameter information on cellular features and oxidative stress damage within single …

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WebApr 12, 2024 · Cell viability and proliferation were determined using CCK‑8 assay and EdU staining. Flow cytometry was used to determine cell death and cell cycle arrest. Intracellular lipid reactive oxygen species, Fe2+ and mitochondrial membrane potential (MMP) were analyzed using a C11‑BODIPY581/591 fluorescent probe, FerroOrange staining and a … WebThe staining method of lipid peroxidation sensor C11-BODIPY was carried out as described by [21]. In brief, C11-BODIPY (581/591) (Thermo Fisher) stock solution was prepared in ethanol to 2 mM ...

WebApr 13, 2024 · Oxidation of the poly-unsaturated butadienyl portion of C11-BODIPY resulted in a shift of the fluorescence emission peak from ∼590 to ∼510 nm. Samples were analyzed using flow cytometry (Exc: 488 nm, Em: 510 nm) after washing twice with PBS, and the results were analyzed by NovoExpress software. Detection of Cellular Fe 2+ Ions … WebContent may be subject to copyright. C11-BODIPY (581/591) staining shows lipid peroxidation and membrane trafficking inside the cell to lysosomes. (a) Confocal imaging of C11-BODIPY loaded cells ...

WebMar 8, 2024 · By ferroptosis inducers of erastin, sulfasalazine, and cyst (e)ine deprivation, lipid peroxidation measured using BODIPY™ 581/591 C11, a lipid peroxidation sensor [ 15 ], was significantly increased in HNC cells with DMT1 siRNA compared to siCtr cells ( P < 0.01) (Fig. 1 G). WebJan 15, 2024 · Add 2 μL of 1.5 mM BODIPY-C11 stock solution to “no erastin” and “5 μM erastin” wells. DO NOT add BODIPY-C11 to the “no staining” well (see Notes 8 and 9). …

Web产品简介: FINO2(Ferroptosis inducer) 铁死亡诱导剂. 产品关键词： FINO2；Erastin 爱拉斯汀；Liproxstatin-1；铁死亡抑制剂；(1S,3R)-RSL3；Ferroptosis inducer 铁死亡诱导剂；FeRhoNox-1 (Fe2+indicator) 亚铁离子荧光探针；C11 BODIPY …

WebSep 14, 2024 · Lipid peroxidation, intracellular and mitochondrial iron were detected by Liperfluo, C11-BODIPY 581/591, FerroOrange and Mito-FerroGreen, respectively. We … birmingham performing arts schoolWebFerroptosis and Zinc homeostasis pathway enrichment are shown. c Table of the top-4 pathways enriched in Enrichr analysis and the corresponding genes involved. d C11 … birmingham personal injury law firmsWebDec 29, 2024 · FerroOrange was purchased from Dojindo Laboratories (Japan). The bicinchoninic acid protein assay kit, Lipid Peroxidation Fluorescent Probe (C11 BODIPY … birmingham pest control ray wallaceWebDec 29, 2024 · FerroOrange was purchased from Dojindo Laboratories (Japan). The bicinchoninic acid protein assay kit, Lipid Peroxidation Fluorescent Probe (C11 BODIPY 581/591), mitoTracker Red and Tetramethylrhodamine methyl ester (TMRM) fluorescence probes were obtained from Thermo Fisher Scientific (Waltham, MA). dangerous country to visitWebAug 13, 2013 · Histograms and pictures showing the changes in cellular green (A), orange (B), and red fluorescence (C) before and after staining with C11-BODIPY 581/591 in unexposed algae and exposed to 5 × 10 … dangerous crush crystal kaswellWebJul 1, 2024 · By staining cells with FerroOrange (to detect ferrous ions), ... **p < 0.01. (F) Intracellular LOS levels by C11-BODIPY staining, (G) ferrous staining by FerroOrange … dangerous country in asiaWebNov 12, 2024 · After 24 h, these cells were co-cultured with 1 mL of fresh DMEM comprising 5 μM C11-BODIPY 581/591 (cat. GC40165, GLPBIO, Montclair, CA, USA) at 37 °C for 30 min. Subsequently, cells were subjected to washing twice with PBS, harvested with trypsinization, washed again and kept in resuspension of 400 μL of PBS buffer … dangerous country for women in the world